RNA interference with FOXL2 and DMRT1

Overview
RNA interference is a technique to silence the transcription of selected genes, by producing an RNA strand complimentary to the mRNA transcript of the selected gene which will be recognized as belonging to an invading virus by the body, and blocked from getting to the ribosome. SNALPs are lipid membranes which contain copies of siRNA. When they hit a cell, they fuse with the cell membrane, disbursing the interfering RNA into the cell.

Synthesis Steps

 * Identify interfering siRNA sequence
 * Acquire DNA for siRNA
 * RNA polymerase to amplify siRNA
 * SNALP formation
 * Sonication to embed siRNA within SNALP
 * Safety checks on siRNA purity

Research Wishlist

 * SNALP preparation protocol
 * Protocols used by FDA trials of RNAi
 * Previous roadblocks with RNAi